Author:
Hou Yiling,Chai Cijing,Wang Shufang
Abstract
Objective To explore the effect of HIF-1α on the cardiovascular protective effect of Astragaloside IV. Method To divide the mice into four groups, hypertension model group (LC), Astragaloside IV treatment group (LR), HIF-1α overexpression combined with Astragaloside IV treatment group (LO), HIF-1α knockdown combined with Astragaloside IV treatment group (LK), and measure the mean blood pressure of mice at day 1, day 7, day 14 and day 21. Then, the aortic tissue sample was collected, the expression of HIF-1α related signaling pathway molecules were detected using western blot and qPCR method. The concentration of vascular protective molecules was detected using ELISA method. Results The mean blood pressure in LO group was significantly decreased, while the JAK/STAT3/HIF-1α signaling pathway was significantly activated, and the expression of anti-apoptosis and vascular protection molecules was increased. Besides, the concentration of vascular protection molecules was significantly increased in blood sample. Conclusions Overexpression of HIF-1α significantly activated the JAK/STAT3 signaling pathway, increased the expression and the concentration of vascular protection related molecules, enhanced the cardiovascular protective effect of Astragaloside IV.
Publisher
Polish Pharmaceutical Society
Subject
Pharmaceutical Science,Pharmacology