Determination of Total Biotin by Liquid Chromatography Coupled with Immunoaffinity Column Cleanup Extraction: Multilaboratory Testing, Final Action 2016.02
Author:
Joseph George1, Devi Ranjani1, Marley Elaine C2, Leeman David2, Bhandari S, Butler Thompson L, Campos Giminez E, Chen H, Dekker N, Devi R, Dole M, Gallegos-Peretz T, Gill B, Gill T, Green Y, Hodgson M, Huo X, Indyk H, Joseph G, Kennedy L, Leeman D, Lim F, Macfadzean C, McMahon A, Malaviole I, Marley E, Seegers M, Schimpf K, Wang J, Wollard D, Yadlapalli S, Yin C,
Affiliation:
1. AsureQuality Ltd, PO Box 41, Shortland St, Auckland 1140, New Zealand 2. R-Biopharm Rhône Ltd, West of Scotland Science Park, Glasgow G20 0XA, United Kingdom
Abstract
Abstract
Single- and multilaboratory testing data have provided systematic scientific evidence that a simple, selective, accurate, and precise method can be used as a potential candidate reference method for dispute resolution in determining total biotin in all forms of infant, adult, and/or pediatric formula. Using LC coupled with immunoaffinity column cleanup extraction, the method fully meets the intended purpose and applicability statement in AOAC Standard Method Performance Requirement 2014.005. The method was applied to a cross-section of infant formula and adult nutritional matrixes, and acceptable precision and accuracy were established. The analytical platform is inexpensive, and the method can be used in almost any laboratory worldwide with basic facilities. The immunoaffinity column cleanup extraction is the key step to successful analysis.
Publisher
Oxford University Press (OUP)
Subject
Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry
Cited by
2 articles.
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