Quantitative Determination of Anthraquinones and Resveratrol in Polygonum Cillinerve (Nakai) Ohwi by HPLC-PAD

Abstract

Abstract A simple, sensitive, and validated high-performance liquid chromatography-photodiode array method has been established for the simultaneous determination of anthraquinones and resveratrol in Polygonum Cillinerve (Nakai) Ohwi (Zhushaqi in Chinese). The evaluation was performed using a Sunfire C18 reversed-phase column with 30°C column temperature. The mobile phase was composed of a gradient elution of 0.5% acetic acid (solvent A) and methanol (solvent B) with flow rate of 1.0 mL/min. The detection wavelength was at 254nm. The method developed and validated is simple, shows good linearity, sensitivity, precision, and recovery, and is applied to analyze anthraquinones and resveratrol in 13 batches of Zhushaqi. The results show that the target compounds of Zhushaqi are significantly different among these samples. Based on results of the statistical analysis, the samples collected from Funiu Mountain were clustered together, and the samples obtained fromBozhou Market were close together. The developed method can be a useful tool in quality control and used to evaluate difference and to identify the geographical area of Zhushaqi, and also to provide technical support for the pharmacological and clinical research of related drugs.