Application of Mobile Phases Containing Ionic Liquid for HPLC Analysis of Selected Isoquinoline Alkaloids-Reference-Cited by-同舟云学术

Application of Mobile Phases Containing Ionic Liquid for HPLC Analysis of Selected Isoquinoline Alkaloids

Published:2017-11-01 Issue:6 Volume:100 Page:1652-1659
ISSN:1060-3271
Container-title:Journal of AOAC INTERNATIONAL
language:en
Short-container-title:

Author:

Petruczynik Anna¹,Misiurek Justyna¹,Tuzimski Tomasz²,Waksmundzka-Hajnos Monika¹

Affiliation:

1. Medical University of Lublin, Department of Inorganic Chemistry, Chodźki 4a, 20-093 Lublin, Poland

2. Medical University of Lublin, Department of Physical Chemistry, Chodźki 4a, 20-093 Lublin, Poland

Abstract

Abstract An HPLC procedure on a polar reversed-phase column with mobile phases containing ionic liquid (IL) was developed for the analysis of selected alkaloids from different chemical groups. We aimed to obtain optimal conditions for the separation of alkaloids because widely used silica-based stationary phases exhibit a silanol effect, rendering analysis of basic analytes extremely difficult. Retention, separation selectivity, peak symmetry, and system efficiency were examined in various eluent systems containing different concentrations of IL and acetonitrile. The obtained results revealed substantial influence from the concentrations of IL, the organic modifier, and temperature on the retention behavior of the investigated alkaloids. The most selective and efficient chromatographic systems were applied for the analysis of several alkaloids in a plant extract.

Publisher

Oxford University Press (OUP)

Subject

Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry

Link

http://academic.oup.com/jaoac/article-pdf/100/6/1652/32426671/jaoac1652.pdf

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