Determination of Corticosteroids, Anabolic Steroids, and Basic Nonsteroidal Anti-Inflammatory Drugs in Milk and Animal Tissues

Abstract

Abstract A quantitative LC/MS/MS method was developed for the determination of 14 steroidal compounds and three basic nonsteroidal anti-inflammatory drugs (detected as metabolites) in bovine milk and animal muscle tissue. The proposed method is sufficiently sensitive and highly selective for residueapplications. The described approach offers the possibility to detect, quantify, and confirm anti-inflammatory drugs belonging to two widely diverging chemical categories. The employed single-stage SPE step (mixed mode cation exchange) retains both steroids and basic metabolites of nonsteroidal anti-inflammatory drugs. The method is capableof handling widely diverging relevant concentration ranges (0.1 μg/kg for dexamethasone and100 μg/kg for metamizol metabolites) for the individual compounds with a single extraction,cleanup, and LC/MS/MS procedure. It provides good analyte precision and accuracy data.