Evaluation of the VITEK 2 Gram-Negative (GN) Microbial Identification Test Card: Collaborative Study

Author:

Crowley Erin1,Bird Patrick1,Fisher Kiel1,Goetz Katherine1,Boyle Megan1,Benzinger, Jr M Joseph1,Juenger Marc1,Agin James1,Goins David1,Johnson Ronald2,Canale J,Cherney D,Coakley M,Colón-Reveles J,Crowe J,Daugherty R,Dombroski P,Dwivedi H P,Dyer J,Elems C,Finnigan J,Fraser G,Garza A,Glover J,Hanson P,Hayman M,High E,Johnson L,Kelly M,Kim S,Kim S G,Kingsley M,McDonough M,Miller A,Mills J,Montez S,Murphy M,Parra G,Patel U,Robertson L,Robeson S,Saldana A,Salfnger Y,Segarra M,Shaw B,Sidebottom T,Toney D,Wang H,Wikoff P,Wilson K,

Affiliation:

1. Q Laboratories, Inc., 1400 Harrison Ave, Cincinnati, OH 45214

2. bioMérieux, Inc., 595 Anglum Rd, Hazelwood, MO 63042

Abstract

Abstract A collaborative study was conducted to evaluate the performance of the VITEK® 2 Gram-Negative (GN) Identification card for use with the VITEK 2 automated microbial identification system. The GN test card is used in the identification of fermenting and nonfermenting Gram-negative bacilli, including the select agent organisms Brucella melitensis, Francisella tularensis, Burkholderia mallei, B. pseudomallei, and Yersinia pestis. The VITEK 2 GN card is based on 47 biochemical tests measuring carbon source utilization, inhibition and resistance, and enzymatic activities. A total of 20 laboratories representing government, industry, and private testing facilities throughout the United States participated. In this study, 720 Gram-negative inclusivity isolates were analyzed by the GN Identification method. Of the 720 well-characterized isolates, 707 were identified correctly, 0 were misidentified, 0 were unidentified, and 13 were not characterized as a Gram-negative organism. Additionally, 120 isolates exclusive of fermenting and nonfermenting Gram-negative bacilli were screened by Gram stain. A total of 117 isolates were correctly excluded. Three organisms were incorrectly characterized by Gram stain procedures, resulting in incorrect analysis and misidentification by VITEK 2 GN. The VITEK 2 GN identification method is an acceptable automated method for the rapid identification of Gram-negative bacteria.

Publisher

Oxford University Press (OUP)

Subject

Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry

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