Evaluation of the VITEK 2 Gram-Negative (GN) Microbial Identification Test Card: Collaborative Study
Author:
Crowley Erin1, Bird Patrick1, Fisher Kiel1, Goetz Katherine1, Boyle Megan1, Benzinger, Jr M Joseph1, Juenger Marc1, Agin James1, Goins David1, Johnson Ronald2, Canale J, Cherney D, Coakley M, Colón-Reveles J, Crowe J, Daugherty R, Dombroski P, Dwivedi H P, Dyer J, Elems C, Finnigan J, Fraser G, Garza A, Glover J, Hanson P, Hayman M, High E, Johnson L, Kelly M, Kim S, Kim S G, Kingsley M, McDonough M, Miller A, Mills J, Montez S, Murphy M, Parra G, Patel U, Robertson L, Robeson S, Saldana A, Salfnger Y, Segarra M, Shaw B, Sidebottom T, Toney D, Wang H, Wikoff P, Wilson K,
Affiliation:
1. Q Laboratories, Inc., 1400 Harrison Ave, Cincinnati, OH 45214 2. bioMérieux, Inc., 595 Anglum Rd, Hazelwood, MO 63042
Abstract
Abstract
A collaborative study was conducted to evaluate the performance of the VITEK® 2 Gram-Negative (GN) Identification card for use with the VITEK 2 automated microbial identification system. The GN test card is used in the identification of fermenting and nonfermenting Gram-negative bacilli, including the select agent organisms Brucella melitensis, Francisella tularensis, Burkholderia mallei, B. pseudomallei, and Yersinia pestis. The VITEK 2 GN card is based on 47 biochemical tests measuring carbon source utilization, inhibition and resistance, and enzymatic activities. A total of 20 laboratories representing government, industry, and private testing facilities throughout the United States participated. In this study, 720 Gram-negative inclusivity isolates were analyzed by the GN Identification method. Of the 720 well-characterized isolates, 707 were identified correctly, 0 were misidentified, 0 were unidentified, and 13 were not characterized as a Gram-negative organism. Additionally, 120 isolates exclusive of fermenting and nonfermenting Gram-negative bacilli were screened by Gram stain. A total of 117 isolates were correctly excluded. Three organisms were incorrectly characterized by Gram stain procedures, resulting in incorrect analysis and misidentification by VITEK 2 GN. The VITEK 2 GN identification method is an acceptable automated method for the rapid identification of Gram-negative bacteria.
Publisher
Oxford University Press (OUP)
Subject
Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry
Cited by
36 articles.
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