PDLIM2 can inactivate the TGF‐β/Smad pathway to inhibit the malignant behavior of ovarian cancer cells

Abstract

AbstractPDZ‐LIM domain‐containing Protein 2 (PDLIM2) has been reported to be downregulated in ovarian cancer. However, its exact function and mechanism in regulating ovarian cancer progression have not been elucidated. This work researched the exert effect and mechanism of PDLIM2 on ovarian cancer progression. Briefly, PDLIM2 expression in clinical tissues of ovarian cancer patients and cells was investigated by qRT‐PCR and Western blot. The function of PDLIM2 on the proliferation, colony formation, migration and invasion of ovarian cancer cells was explored via cell counting kit‐8, colony formation and Transwell assays. To verify whether PDLIM2 regulates ovarian cancer progression via regulating the transforming growth factor‐β (TGF‐β)/Smad pathway, exogenous TGF‐β (10 ng/mL) treatment was performed on the PDLIM2‐overexpressed ovarian cancer cells. PDLIM2 effect on the in vivo growth of ovarian cancer cells was researched by establishing a xenograft tumor model. Immunohistochemistry and Western blot were performed to protein expression in cells and tissues. As a result, PDLIM2 was low‐expressed in ovarian cancer tissues/cells. PDLIM2 upregulation attenuated the proliferation, colony formation, migration, invasion and epithelial‐mesenchymal transition (EMT) of ovarian cancer cells, and inactivated the TGF‐β/Smad pathway. The opposite results were found in the PDLIM2‐silenced ovarian cancer cells. Exogenous TGF‐β treatment abrogated the inhibition of PDLIM2 on the malignant behavior of ovarian cancer cells. PDLIM2 upregulation attenuated the in vivo growth and EMT of ovarian cancer cells. Thus, PDLIM2 attenuates the proliferation, migration, invasion and EMT of ovarian cancer cells via inactivating the TGF‐β/Smad pathway. PDLIM2 may be a usefully target for ovarian cancer treatment.