Effects of Follicular Fluid Administration on Serum Bioactive and Immunoreactive FSH Concentrations and Compensatory Testosterone Secretion in Hemicastrated Adult Rats

Abstract

ABSTRACT: In adult rats, removal of one testis (hemicastration) results in an elevation of serum follicle‐stimulating hormone (FSH) concentrations and a compensation in testosterone secretion by the remaining testis without a corresponding increase in testis size. To determine whether changes in FSH secretion and compensatory androgen production are related, serum testosterone concentrations were measured after inhibin‐rich porcine follicular fluid was administered twice daily for 4 days to block the hemicastration‐induced rise in FSH. Both serum immunoreactive FSH (immuno‐FSH) and bioactive FSH (bio‐FSH) concentrations were increased 4 days after hemicastration. The significant increase in serum immuno‐FSH in hemicastrated animals was prevented by follicular fluid administration, whereas the serum bio‐FSH activity and biologic to immunologic (B/l) ratios were increased in follicular fluid‐treated animals. The follicular fluid‐induced reduction in serum immuno‐FSH had no effect on serum testosterone secretion in hemicastrated rats. Serum inhibin concentrations were reduced 27% in hemicastrated rats compared with intact controls, while administration of exogenous follicular fluid increased serum inhibin concentrations. An elevation in serum immuno‐FSH secretion after hemicastration apparently is not required for the compensatory testosterone response. However, the observation of increased bio‐FSH in hemicastrated and follicular fluid‐treated animals raises questions about the importance of FSH quality (bioactivity), rather than quantity, for controlling testicular steroidogenic activity.