MicroRNA‐20a in extracellular vesicles derived from duodenal fluid is a possible biomarker for pancreatic ductal adenocarcinoma

Author:

Taniguchi Takashi1ORCID,Ideno Noboru1,Araki Tomoyuki1,Miura Shun1,Yamamoto Masahiro1,Nakafusa Tomoki1,Higashijima Nobuhiro1,Yamamoto Takeo2,Tamura Koji1,Nakamura So1,Abe Toshiya1,Ikenaga Naoki1,Nakata Kohei1,Ohuchida Kenoki1,Oda Yoshinao2,Ohtsuka Takao3,Nakamura Masafumi1

Affiliation:

1. Department of Surgery and Oncology Graduate School of Medical Sciences Kyushu University Fukuoka Japan

2. Department of Anatomic Pathology Graduate School of Medical Sciences Kyushu University Fukuoka Japan

3. Department of Digestive Surgery Breast and Thyroid Surgery Graduate School of Medical and Dental Sciences Kagoshima University Kagoshima Japan

Abstract

AbstractBackgroundPancreatic ductal adenocarcinoma (PDAC) has a high mortality rate owing to its late diagnosis and aggression. In addition, there are relatively few minimally invasive screening methods for the early detection of PDAC, making the identification of biomarkers for this disease a critical priority. Recent studies have reported that microRNAs in extracellular vesicles (EV‐miRs) from bodily fluids can be useful for the diagnosis of PDACs. Given this, we designed this study to evaluate the utility of cancer EVs extracted from duodenal fluid (DF) and their resident EV‐miRs as potential biomarkers for the detection of PDAC.MethodsEV‐miRs were evaluated and identified in the supernatants of various pancreatic cancer cell lines (Panc‐1, SUIT2, and MIAPaca2), human pancreatic duct epithelial cells, and the DF from patients with PDAC and healthy controls. EVs were extracted using ultracentrifugation and the relative expression of EV‐miR‐20a was quantified.ResultsWe collected a total of 34 DF samples (27 PDAC patients and seven controls) for evaluation and our data suggest that the relative expression levels of EV‐miR‐20a were significantly higher in patients with PDAC than in controls (p = 0.0025). In addition, EV‐miR‐20a expression could discriminate PDAC from control patients regardless of the location of the tumor with an area under the curve values of 0.88 and 0.88, respectively.ConclusionsWe confirmed the presence of EVs in the DF and suggest that the expression of EV‐miR‐20a in these samples may act as a potential diagnostic biomarker for PDAC.

Publisher

Wiley

Reference33 articles.

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