Caged Dexamethasone to Photo‐control the Development of Embryos through Activation of the Glucocorticoid Receptor-Reference-Cited by-同舟云学术

Caged Dexamethasone to Photo‐control the Development of Embryos through Activation of the Glucocorticoid Receptor

Published:2024-02-29 Issue:23 Volume:30 Page:
ISSN:0947-6539
Container-title:Chemistry – A European Journal
language:en
Short-container-title:Chemistry A European J

Author:

Mandal Mrinal¹^ORCID,Scerbo Pierluigi²^ORCID,Coghill Ian¹^ORCID,Riou Jean‐François³^ORCID,Bochet Christian G.⁴^ORCID,Ducos Bertrand²⁵^ORCID,Bensimon David²⁶,Le Saux Thomas¹^ORCID,Aujard Isabelle¹,Jullien Ludovic¹^ORCID

Affiliation:

1. PASTEUR Département de chimie École Normale Supérieure PSL University Sorbonne Université, CNRS 24, rue Lhomond 75005 Paris France

2. Laboratoire de Physique de l'Ecole Normale Supérieure Paris Sciences Lettres University Sorbonne Université Université de Paris, Centre National de la Recherche Scientifique 24 Rue Lhomond 75005 Paris France

3. Laboratory of Developmental Biology Institute of Biology Paris-Seine Sorbonne University, CNRS 9, Quai Saint-Bernard 75252 Paris, Cedex 05 France

4. Department of Chemistry University of Fribourg 9 Ch. du Musée CH-1700 Fribourg Switzerland

5. High Throughput qPCR Core Facility Ecole Normale Supérieure Paris Sciences Lettres University 46 Rue d'Ulm 75005 Paris France

6. Department of Chemistry and Biochemistry University of California Los Angeles CA 90095 USA

Abstract

AbstractEfficient tools for controlling molecular functions with exquisite spatiotemporal resolution are much in demand to investigate biological processes in living systems. Here we report an easily synthesized caged dexamethasone for photo‐activating cytoplasmic proteins fused to the glucocorticoid receptor. In the dark, it is stable in vitro as well as in vivo in both zebrafish (Danio rerio) and Xenopus sp, two significant models of vertebrates. In contrast, it liberates dexamethasone upon UV illumination, which has been harnessed to interfere with developmental steps in embryos of these animals. Interestingly, this new system is biologically orthogonal to the one for photo‐activating proteins fused to the estrogen ERT receptor, which brings great prospect for activating two distinct proteins down to the single cell level.

Funder

Inserm Transfert

Publisher

Wiley

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