Functional Plasticity of a Viral Terpene Synthase, OILTS, that Shows Non–Specific Metal Cofactor Binding and Metal–Dependent Biosynthesis

Author:

Jung Youngcheol1ORCID,Mitsuhashi Takaaki2ORCID,Kikuchi Takashi3,Fujita Makoto124ORCID

Affiliation:

1. Department of Applied Chemistry School of Engineering The University of Tokyo Mitsui Link Lab Kashiwanoha 1, FS CREATION, 6–6-2 Kashiwanoha Kashiwa Chiba 277-0882 Japan

2. Division of Advanced Molecular Science Institute for Molecular Science (IMS) 5-1 Higashiyama, Myodaiji Okazaki Aichi 444-8787 Japan

3. Rigaku Corporation 3–9-12 Matsubaracho Akishima Tokyo 196-8666 Japan

4. Tokyo College Institutes for Advanced Study The University of Tokyo Mitsui Link Lab Kashiwanoha 1, FS CREATION, 6–6-2 Kashiwanoha Kashiwa Chiba 277-0882 Japan

Abstract

AbstractOILTS is a viral class I terpene synthase found from the giant virus Orpheovirus IHUMI–LCC2. It exhibits a unique structure and demonstrates high plasticity to metal cofactors, allowing it to biosynthesize different cyclic terpene frameworks. Notably, while OILTS produces only (+)‐germacrene D–4–ol with the most common cofactor, Mg2+, it also biosynthesizes a different cyclic terpene, (+)‐cubebol, with Mn2+, Co2+, or Ni2+, presenting a rare instance of cofactor‐dependent enzyme catalysis. This is the first report of (+)‐cubebol biosynthesis, to our knowledge. In addition, OILTS can uptake Zn2+ as a cofactor, which is uncommon among ordinary terpene synthases. These findings suggest that OILTS′s functional plasticity may benefit the virus in diverse host environments, highlighting potential evolutionary implications.

Funder

Japan Society for the Promotion of Science

Publisher

Wiley

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3