Programmed Control of Fluorescence Blinking Patterns based on Electron Transfer in DNA

Author:

Fan Shuya1,Takada Tadao2,Maruyama Atsushi3ORCID,Fujitsuka Mamoru1ORCID,Kawai Kiyohiko3ORCID

Affiliation:

1. SANKEN (The Institute of Scientific and Industrial Research) Osaka University Mihogaoka 8-1 Ibaraki Osaka 567-0047 Japan

2. Department of Applied Chemistry Graduate School of Engineering University of Hyogo 2167 Shosha Himeji Hyogo 671-2280 Japan

3. Department of Life Science and Technology Tokyo Institute of Technology 4259 B-57 Nagatsuta, Midori-ku Yokohama Kanagawa 226-8501 Japan

Abstract

AbstractFluorescence imaging uses changes in the fluorescence intensity and emission wavelength to analyze multiple targets simultaneously. To increase the number of targets that can be identified simultaneously, fluorescence blinking can be used as an additional parameter. To understand and eventually control blinking, we used DNA as a platform to elucidate the processes of electron transfer (ET) leading to blinking, down to the rate constants. With a fixed ET distance, various blinking patterns were observed depending on the DNA sequence between the donor and acceptor units of the DNA platform. The blinking pattern was successfully described with a combination of ET rate constants. Therefore, molecules with various blinking patterns can be developed by tuning ET. It is expected that the number of targets that can be analyzed simultaneously will increase by the power of the number of blinking patterns.

Funder

Precursory Research for Embryonic Science and Technology

Publisher

Wiley

Subject

General Chemistry,Catalysis,Organic Chemistry

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