Synthesis of DNA‐Boron Cluster Composites and Assembly into Functional Nanoparticles with Dual, Anti‐EGFR, and Anti‐c‐MYC Oncogene Silencing Activity-Reference-Cited by-同舟云学术

Synthesis of DNA‐Boron Cluster Composites and Assembly into Functional Nanoparticles with Dual, Anti‐EGFR, and Anti‐c‐MYC Oncogene Silencing Activity

Published:2024-01-24 Issue:14 Volume:30 Page:
ISSN:0947-6539
Container-title:Chemistry – A European Journal
language:en
Short-container-title:Chemistry A European J

Author:

Bednarska‐Szczepaniak Katarzyna¹^ORCID,Ebenryter‐Olbińska Katarzyna²^ORCID,Gajek Gabriela³^ORCID,Śmiałkowski Krzysztof¹⁴^ORCID,Suwara Justyna²^ORCID,Fiedorowicz Lidia⁵⁴^ORCID,Leśnikowski Zbigniew¹

Affiliation:

1. Laboratory of Medicinal Chemistry Institute of Medical Biology Polish Academy of Sciences Lodowa Łódź, 106 92-232 Lodz Poland

2. Centre of Molecular and Macromolecular Studies Polish Academy of Sciences Sienkiewicza Łódź, 112 90-363 Lodz Poland

3. Laboratory of Immunobiology of Infections Institute of Medical Biology Polish Academy of Sciences Lodowa Łódź, 106 92-232 Lodz Poland

4. Lodz Institutes of the Polish Academy of Science The Bio-Med-Chem Doctoral School University of Lodz Poland

5. Laboratory of Mycobacterium Genetics and Physiology Institute of Medical Biology Polish Academy of Sciences Lodowa Łódź, 106 92-232 Lodz Poland

Abstract

AbstractA versatile method for the automated synthesis of composites containing DNA‐oligonucleotides and boron cluster scaffolds and their assembly into functional nanoparticles is described. The obtained, torus‐like nanoparticles carry antisense oligonucleotides that target two different oncogenes simultaneously. The nanoparticles exhibited notable silencing efficiency in vitro in a pancreatic carcinoma cell line PANC‐1 toward EGFR and c‐Myc genes at the mRNA level, and a significant efficiency at the protein level. The proposed approach may be an attractive alternative to methods currently used, including one therapeutic nucleic acid, one genetic target, or the use of cocktails of therapeutic nucleic acids.

Funder

Narodowe Centrum Nauki

Ministerstwo Edukacji i Nauki

Publisher

Wiley

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