Aptamer Clicked Poly(ferrocenylsilanes) at Au Nanoparticles as Platforms with Multiple Function[†]

Author:

Halmagyi Tibor G.1ORCID,Alsharif Nizar B.2ORCID,Berkal Mohamed A.1ORCID,Hempenius Mark A.3,Szilagyi Istvan2ORCID,Vancso G. Julius3ORCID,Nardin Corinne1ORCID

Affiliation:

1. E2S UPPA, CNRS, IPREM Universite de Pau et des Pays de l'Adour F- 64053 Pau France

2. MTA-SZTE Momentum Biocolloids Research Group Department of Physical Chemistry and Materials Science Interdisciplinary Research Center University of Szeged H- 6720 Szeged Hungary

3. Sustainable Polymer Chemistry University of Twente NL- 7522NB Enschede the Netherlands

Abstract

AbstractAptamers are widely used in biosensing due to their specific sensitivity toward many targets. Thus, gold nanoparticle (AuNP) aptasensors are subject to intense research due to the complementary properties of aptamers as sensing elements and AuNPs as transducers. We present herein a novel method for the functional coupling of thrombin‐specific aptamers to AuNPs via an anionic, redox‐active poly(ferrocenylsilane) (PFS) polyelectroyte. The polymer acts as a co‐reductant and stabilizer for the AuNPs, provides grafting sites for the aptamer, and can be used as a redox sensing element, making the aptamer‐PFS‐AuNP composite (aptamer‐AuNP) a promising model system for future multifunctional sensors. The aptamer‐AuNPs exhibit excellent colloidal stability in high ionic strength environments owing to the combined electrosteric stabilizing effects of the aptamer and the PFS. The synthesis of each assembly element is described, and the colloidal stability and redox responsiveness are studied. As an example to illustrate applications, we present results for thrombin sensitivity and specificity using the specific aptamer.

Funder

Centre National de la Recherche Scientifique

Agence Nationale de la Recherche

Magyar Tudományos Akadémia

Publisher

Wiley

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