Affiliation:
1. Department of Applied Chemistry Faculty of Science and Engineering Chuo University 1-13-27 Kasuga Bunkyo-ku Tokyo 112-8551 Japan
2. Department of General Thoracic Surgery Saitama Medical Center Saitama Medical University 1981 Kamoda, Kawagoe-shi Saitama 350-8550 Japan
Abstract
AbstractMyoglobin combined with human serum albumin (Mb‐HSA) can be produced using yeast Pichia pastoris as a host strain, with secretion into the culture medium. This Mb‐HSA fusion protein possesses identical O2 binding affinity to that of naked Mb. The Mb unit is reconstituted with a zinc(II) protoporphyrin IX, yielding (zinc substituted Mb)‐HSA, ZnMb‐HSA. The photophysical property and singlet O2 generation ability of ZnMb‐HSA are equivalent to those of ZnMb. In vitro cell experiments revealed that ZnMb‐HSA acts as a superior photosensitizer for photodynamic cancer therapy. It is noteworthy that ZnMb‐HSA shows long circulation lifetime in vivo.
Funder
Promotion and Mutual Aid Corporation for Private Schools of Japan
Japan Society for the Promotion of Science
Chuo University
Subject
General Chemistry,Catalysis,Organic Chemistry
Cited by
2 articles.
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