Dioxygen Binding Is Controlled by the Protein Environment in Non‐heme FeII and 2‐Oxoglutarate Oxygenases: A Study on Histone Demethylase PHF8 and an Ethylene‐Forming Enzyme

Author:

Chaturvedi Shobhit S.1,Thomas Midhun George1,Rifayee Simahudeen Bathir Jaber Sathik1,White Walter1,Wildey Jon2,Warner Cait3,Schofield Christopher J.4,Hu Jian56,Hausinger Robert P.57,Karabencheva‐Christova Tatayana G.1,Christov Christo Z.1ORCID

Affiliation:

1. Department of Chemistry Michigan Technological University Houghton MI-49931 USA

2. Department of Chemical Engineering Michigan Technological University Houghton MI-49931 USA

3. Department of Biology Michigan Technological University Houghton MI-49931 USA

4. The Chemistry Research Laboratory Department of Chemistry and the Ineos Oxford Institute for Antimicrobial Research University of Oxford Mansfield Road Oxford OX1 3TA UK

5. Department of Biochemistry and Molecular Biology Michigan State University East Lansing MI-48824 USA

6. Department of Chemistry Michigan State University East Lansing MI-48824 USA

7. Department of Microbiology and Molecular Genetics Michigan State University East Lansing MI-48824 USA

Abstract

AbstractThis study investigates dioxygen binding and 2‐oxoglutarate (2OG) coordination by two model non‐heme FeII/2OG enzymes: a class 7 histone demethylase (PHF8) that catalyzes the hydroxylation of its H3K9me2 histone substrate leading to demethylation reactivity and the ethylene‐forming enzyme (EFE), which catalyzes two competing reactions of ethylene generation and substrate l‐Arg hydroxylation. Although both enzymes initially bind 2OG by using an off‐line 2OG coordination mode, in PHF8, the substrate oxidation requires a transition to an in‐line mode, whereas EFE is catalytically productive for ethylene production from 2OG in the off‐line mode. We used classical molecular dynamics (MD), quantum mechanics/molecular mechanics (QM/MM) MD and QM/MM metadynamics (QM/MM‐MetD) simulations to reveal that it is the dioxygen binding process and, ultimately, the protein environment that control the formation of the in‐line FeIII‐OO⋅ intermediate in PHF8 and the off‐line FeIII‐OO⋅ intermediate in EFE.

Funder

Division of Chemistry

National Institute of General Medical Sciences

Publisher

Wiley

Subject

General Chemistry,Catalysis,Organic Chemistry

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