Affiliation:
1. Laboratoire de Bioimagerie et Pathologies UMR 7021 CNRS/Université de Strasbourg 74 route du Rhin 67401 Illkirch-Graffenstaden France
Abstract
AbstractDual‐emissive photoconvertible fluorophores (DPCFs) are powerful tools to unambiguously track labeled cells in bioimaging. We recently introduced a new rational mechanism called directed photooxidation‐induced conversion (DPIC) enabling efficient DPCFs to be obtained by conjugating a coumarin to aromatic singlet‐oxygen reactive moieties (ASORMs). Pyrrole was found to be a suitable ASORM as it provided a high hypsochromic shift along with a fast and efficient conversion. By synthesizing various pyrrole‐based styryl coumarin dyes, we showed that the photoconversion properties, including the quantum yield of photoconversion and the chemical yield of conversion can be tuned by chemical modification of the pyrrole. These modifications led to an improved dual emissive converter, SCP−Boc, which displayed a high brightness and an enhanced photoconversion yield of 63 %. SCP−Boc was successfully used to sequentially photoconvert cells by laser scanning confocal microscopy.
Funder
Agence Nationale de la Recherche
Subject
General Chemistry,Catalysis,Organic Chemistry
Cited by
3 articles.
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