A single-step FACS sorting strategy in conjunction with fluorescent vital dye imaging efficiently assures clonality of biopharmaceutical production cell lines-Reference-Cited by-同舟云学术

A single-step FACS sorting strategy in conjunction with fluorescent vital dye imaging efficiently assures clonality of biopharmaceutical production cell lines

Published:2017-05-04 Issue:6 Volume:12 Page:1700002
ISSN:1860-6768
Container-title:Biotechnology Journal
language:en
Short-container-title:Biotechnol. J.

Author:

Fieder Jürgen¹,Schulz Patrick²,Gorr Ingo¹,Bradl Harald²,Wenger Till³

Affiliation:

1. Early Stage Bioprocess Development; Boehringer Ingelheim GmbH & Co. KG; Biberach Germany

2. Cell Culture Development CMB; Boehringer Ingelheim GmbH & Co. KG; Biberach Germany

3. Global NBE/Biosimilar CMC experts; Boehringer Ingelheim GmbH & Co. KG; Biberach Germany

Publisher

Wiley

Subject

Molecular Medicine,Applied Microbiology and Biotechnology,General Medicine

Reference9 articles.

1. European Medicines Agency Guideline on development, production, characterisation and specification for monoclonal antibodies and related products 2016

2. CHO quasispecies - implications for manufacturing processes;Wurm;Processes,2013

3. Industry view on the relative importance of ”clonality“ of biopharmaceutical-producing cell lines;Frye;Biologicals,2016

4. Poisson statistical analysis of repetitive subcloning by the limiting dilution technique as a way of assessing hybridoma monoclonality;Coller;Methods Enzymol.,1986

5. A statistical approach to determine monoclonality after limiting cell plating of a hybridoma clone;Lietzke;J. Immunol. Methods,1985

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