Addition of urea and thiourea to electrophoresis sample buffer improves efficiency of protein extraction from TCA/acetone-treated smooth muscle tissues for phos-tag SDS-PAGE-Reference-Cited by-同舟云学术

Addition of urea and thiourea to electrophoresis sample buffer improves efficiency of protein extraction from TCA/acetone-treated smooth muscle tissues for phos-tag SDS-PAGE

Published:2017-11-20 Issue:2 Volume:39 Page:326-333
ISSN:0173-0835
Container-title:ELECTROPHORESIS
language:en
Short-container-title:ELECTROPHORESIS

Author:

Takeya Kosuke¹^ORCID,Kaneko Toshiyuki¹,Miyazu Motoi¹,Takai Akira¹

Affiliation:

1. Department of Physiology; Asahikawa Medical University; Hokkaido Japan

Funder

JSPS

Publisher

Wiley

Subject

Clinical Biochemistry,Biochemistry,Analytical Chemistry

Link

http://onlinelibrary.wiley.com/wol1/doi/10.1002/elps.201700394/fullpdf

Reference31 articles.

1. The regulation of protein function by multisite phosphorylation – a 25 year update

2. Filamentous smooth muscle myosin is regulated by phosphorylation.

3. Ca 2+ ‐independent phosphorylation of myosin in rat caudal artery and chicken gizzard myofilaments

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