Affiliation:
1. Department of Women's and Children's Health Karolinska Institutet Stockholm Sweden
2. Astrid Lindgren's Children's Hospital Karolinska University Hospital Stockholm Sweden
3. Thermo Fisher Scientific Uppsala Sweden
4. Department of Women's and Children's Health Uppsala University Uppsala Sweden
5. Division of Immunology and Allergy Department of Medicine Solna Karolinska Institutet and Karolinska University Hospital Stockholm Sweden
Abstract
AbstractBackgroundPreschool wheeze is a risk factor for asthma development. However, the molecular mechanism behind a wheezing episode is not well understood.ObjectiveOur aims were to assess the association of plasma proteins with acute preschool wheeze and to study the proteins with differential expression at the acute phase at revisit after 3 months. Additionally, to investigate the relationship between protein expression and clinical parameters.MethodWe measured 92 inflammatory proteins in plasma and clinical parameters from 145 children during an episode of preschool wheeze (PW) and at the revisit after 3 months (PW‐R, n = 113/145) and 101 healthy controls (HC) aged 6–48 months in the GEWAC cohort using the antibody‐mediated proximity extension‐based assay (Olink Proteomics, Uppsala).ResultsOf the 74 analysed proteins, 52 were differentially expressed between PW and HC. The expression profiles of the top 10 proteins, Oncostatin M (OSM), IL‐10, IL‐6, Fibroblast growth factor 21 (FGF21), AXIN1, CXCL10, SIRT2, TNFSF11, Tumour necrosis factor β (TNF‐β) and CASP8, could almost entirely separate PW from HC. Five out of 10 proteins were associated with intake of oral corticosteroids (OCS) 24 h preceding blood sampling (OSM, CASP8, IL‐10, TNF‐β and CXCL10). No differences in protein expression were seen between PWs with or without OCS in comparison to HC. At the revisit after 3 months, differential protein expressions were still seen between PW‐R and HC for three (IL‐10, SIRT2 and FGF21) of the 10 proteins.ConclusionOur results contribute to unravelling potential immunopathological pathways shared between preschool wheeze and asthma.
Funder
Stiftelsen Konung Gustaf V:s 80-årsfond
Karolinska Institutet
H.K.H. Kronprinsessan Lovisas Förening för Barnasjukvård
Konsul Th C Berghs Stiftelse
Insamlingsstiftelsen Cancer- och Allergifonden
Subject
Immunology and Allergy,Immunology,Pulmonary and Respiratory Medicine
Cited by
1 articles.
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