Towards Synthetic Cells with Self‐Producing Energy

Abstract

AbstractAutonomous generation of energy, specifically adenosine triphosphate (ATP), is critical for sustaining the engineered functionalities of synthetic cells constructed from the bottom‐up. In this mini‐review, we categorize studies on ATP‐producing synthetic cells into three different approaches: photosynthetic mechanisms, mitochondrial respiration mimicry, and utilization of non‐conventional approaches such as exploiting synthetic metabolic pathways. Within this framework, we evaluate the strengths and limitations of each approach and provide directions for future research endeavors. We also introduce a concept of building ATP‐generating synthetic organelle that will enable us to mimic cellular respiration in a simpler way than current strategies. This review aims to highlight the importance of energy self‐production in synthetic cells, providing suggestions and ideas that may help overcome some longstanding challenges in this field.