Targeted Metabolic Investigation of Ligandrol and Analytical Methods Validation for Its Main Long‐term Metabolite-Reference-Cited by-同舟云学术

Targeted Metabolic Investigation of Ligandrol and Analytical Methods Validation for Its Main Long‐term Metabolite

Published:2024-01-22 Issue:5 Volume:89 Page:
ISSN:2192-6506
Container-title:ChemPlusChem
language:en
Short-container-title:ChemPlusChem

Author:

Angelis Yiannis S.¹^ORCID,Sakellariou Panagiotis¹,Petrou Michael²^ORCID,Pitsinos Emmanuel N.³^ORCID

Affiliation:

1. Institute of Biosciences & Applications National Centre for Scientific Research “DEMOKRITOS” Doping Control Laboratory of Athens Neratziotissis & Amaryssias Artemidos Str GR 151 23 Athens Greece

2. Cyprus Anti-Doping Authority Makarion Athletic Centre Avenue, Engomi CY 2400 Nicosia Cyprus

3. Institute of Nanoscience and Nanotechnology National Centre for Scientific Research “DEMOKRITOS” P.O. Box 60037, Aghia Paraskevi GR 153 10 Athens Greece

Abstract

AbstractPrompted by the need for related analytical reference material in the frame of the fight against doping in sports, synthetic efforts towards the main long‐term bishydroxylated metabolite (LGD‐LTM1) of the nonsteroidal selective androgen receptor modulator (SARM) ligandrol have produced related derivatives that were exploited for a targeted metabolite analysis of urine samples obtained in the course of previous excretion studies of this SARM. Further clarifying ligandrol's metabolic profile, the availability of synthetic reference material permitted the structural elucidation of a previously reported pyrrolidinone‐type metabolite and revealed its potential analytical utility as an additional long‐term marker. Moreover, synthetic reference material enabled the comparison and validation of liquid chromatography coupled with mass spectrometry (LC‐MS)‐based and gas chromatography coupled with mass spectrometry (GC‐MS)‐based detection and identification methods focusing on the LGD‐LTM1 marker.

Funder

World Anti-Doping Agency

Publisher

Wiley

Reference27 articles.

1. WADA “International Standard for Education” can be found underhttps://www.wada-ama.org/en/resources/world-anti-doping-program/international-standard-education-ise(accessed 10 October 2023).

2. WADA “2024 List of Prohibited Substances and Methods” can be found underhttps://www.wada-ama.org/en/resources/world-anti-doping-program/2024-prohibited-list(accessed 10 October 2023).

3. WADA “International Standard for Laboratories” can be found underhttps://www.wada-ama.org/en/resources/world-anti-doping-program/international-standard-laboratories-isl(accessed 10 October 2023).

4. WADA “Technical Document – TD2021IDCR” can be found underhttps://www.wada-ama.org/en/resources/lab-documents/td2021idcr(accessed 10 October 2023).

5. Recent advances in identifying and utilizing metabolites of selected doping agents in human sports drug testing