A Photoswitchable Solvatochromic Dye for Probing Membrane Ordering by RESOLFT Super‐resolution Microscopy**-Reference-Cited by-同舟云学术

A Photoswitchable Solvatochromic Dye for Probing Membrane Ordering by RESOLFT Super‐resolution Microscopy**

Published:2023-04-19 Issue:12 Volume:24 Page:
ISSN:1439-4235
Container-title:ChemPhysChem
language:en
Short-container-title:ChemPhysChem

Author:

Frawley Andrew T.¹^ORCID,Leslie Kathryn G.¹^ORCID,Wycisk Virginia¹^ORCID,Galiani Silvia²^ORCID,Shrestha Dilip²^ORCID,Eggeling Christian²³⁴⁵^ORCID,Anderson Harry L.¹^ORCID

Affiliation:

1. Department of Chemistry University of Oxford Chemistry Research Laboratory Mansfield Road Oxford OX1 3TA UK

2. MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine University of Oxford Oxford OX3 9DS UK

3. Institute of Applied Optics and Biophysics Friedrich-Schiller-University Jena Max-Wien-Platz 4 07743 Jena Germany

4. Leibniz Institute of Photonic Technology e.V. Albert-Einstein-Strasse 9 07745 Jena Germany

5. Jena Center for Soft Matter (JCSM) Philosophenweg 7 07743 Jena Germany

Abstract

AbstractA switchable solvatochromic fluorescent dyad can be used to map ordering of lipids in vesicle membranes at a resolution better than the diffraction limit. Combining a Nile Red fluorophore with a photochromic spironaphthoxazine quencher allows the fluorescence to be controlled using visible light, via photoswitching and FRET quenching. Synthetic lipid vesicles of varying composition were imaged with an average 2.5‐fold resolution enhancement, compared to the confocal images. Ratiometric detection was used to probe the membrane polarity, and domains of different lipid ordering were distinguished within the same membrane.

Funder

Deutsche Forschungsgemeinschaft

Wolfson Foundation

Publisher

Wiley

Subject

Physical and Theoretical Chemistry,Atomic and Molecular Physics, and Optics

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/cphc.202300125

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