Affiliation:
1. Khalifa University Chemistry Department and Applied Material Chemistry Center (AMCC) PO Box 127788 Abu Dhabi UAE
Abstract
AbstractD‐Arginine dehydrogenase from Pseudomonas aeruginosa (PaDADH) is an amine oxidase which catalyzes the conversion of D‐arginine into iminoarginine. It contains a non‐covalent FAD cofactor that is involved in the oxidation mechanism. Based on substrate, solvent, and multiple kinetic isotope effects studies, a stepwise hydride transfer mechanism is proposed. It was shown that D‐arginine binds to the active site of enzyme as α‐amino group protonated, and it is deprotonated before a hydride ion is transferred from its α‐C to FAD. Based on a mutagenesis study, it was concluded that a water molecule is the most likely catalytic base responsible from the deprotonation of α‐amino group. In this study, we formulated computational models based on ONIOM method to elucidate the oxidation mechanism of D‐arginine into iminoarginine using the crystal structure of enzyme complexed with iminoarginine. The calculations showed that Arg222, Arg305, Tyr249, Glu87, His 48, and two active site water molecules play key roles in binding and catalysis. Model systems showed that the deprotonation step occurs prior to hydride transfer step, and active site water molecule(s) may have participated in the deprotonation process.
Subject
Physical and Theoretical Chemistry,Atomic and Molecular Physics, and Optics
Cited by
1 articles.
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