Affiliation:
1. Department of Chemistry The University of Texas at Austin 105 E. 24th St. Stop A5300 78712-1224 Austin TX USA
2. Department of Chemistry and Biochemistry University of California, San Diego 9500 Gilman Drive, Natural Sciences Building 3328 92093 La Jolla CA USA
Abstract
AbstractBottom‐up design of biomimetic organelles has gained recent attention as a route towards understanding the transition between non‐living matter and life. Despite various artificial lipid membranes being developed, the specific relations between lipid structure, composition, interfacial properties, and morphology are not currently understood. Sponge‐phase droplets contain dense, nonlamellar lipid bilayer networks that capture the complexities of the endoplasmic reticulum (ER), making them ideal artificial models of such organelles. Here, we combine ultrafast two‐dimensional infrared (2D IR) spectroscopy and molecular dynamics simulations to investigate the interfacial H‐bond networks in sponge‐phase droplets composed of glycolipid and nonionic detergents. In the sponge phase, the interfacial environments are more hydrated and water molecules confined to the nanometer‐scale aqueous channels in the sponge phase exhibit dynamics that are significantly slower compared to bulk water. Surfactant configurations and microscopic phase separation play a dominant role in determining membrane curvature and slow dynamics observed in the sponge phase. The studies suggest that H‐bond networks within the nanometer‐scale channels are disrupted not only by confinement but also by the interactions of surfactants, which extend 1–2 nm from the bilayer surface. The results provide a molecular‐level description for controlling phase and morphology in the design of synthetic lipid organelles.
Funder
U.S. Department of Defense
Multidisciplinary University Research Initiative
Welch Foundation
Subject
Physical and Theoretical Chemistry,Atomic and Molecular Physics, and Optics