Extraction of recombinant periplasmic proteins under industrially relevant process conditions: Selectivity and yield strongly depend on protein titer and methodology-Reference-Cited by-同舟云学术

Extraction of recombinant periplasmic proteins under industrially relevant process conditions: Selectivity and yield strongly depend on protein titer and methodology

Published:2020-04-13 Issue:5 Volume:36 Page:
ISSN:8756-7938
Container-title:Biotechnology Progress
language:en
Short-container-title:Biotechnol Progress

Author:

Schimek Clemens¹,Egger Esther¹,Tauer Christopher¹,Striedner Gerald¹,Brocard Cécile²,Cserjan‐Puschmann Monika¹,Hahn Rainer¹^ORCID

Affiliation:

1. Christian Doppler Laboratory for Production of Next‐Level Biopharmaceuticals in E. coli, Department of Biotechnology University of Natural Resources and Life Sciences Vienna Austria

2. Biopharma Process Science, Boehringer Ingelheim RCV GmbH & Co KG Wien Austria

Funder

Christian Doppler Forschungsgesellschaft

Publisher

Wiley

Subject

Biotechnology

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/btpr.2999

Reference46 articles.

1. Analysis of factors affecting the periplasmic production of recombinant proteins in Escherichia coli;Mergulhao FJ;J Microbiol Biotechnol,2007

2. How to trigger periplasmic release in recombinantEscherichia coli: A comparative analysis