Affiliation:
1. Department of Immunology and Biotechnology University of Pécs, Medical School Pécs Hungary
Abstract
AbstractThe recombinant human proteoglycan aggrecan‐G1 domain (rhG1)‐induced arthritis (GIA) mouse model is a complex model of rheumatoid arthritis (RA). In GIA, autoimmune arthritis is induced by repeated intraperitoneal immunization of genetically susceptible BALB/c mice with the rhG1 antigen emulsified in the adjuvant dimethyldioctadecylammonium (DDA). This article describes the steps for producing and purifying the rhG1 antigen, the immunization protocol, methods for following the clinical picture of arthritis, and the evaluation of relevant laboratory parameters. In this model, the autoimmune arthritis develops stepwise, similar to RA: First is the preclinical stage (after the first immunization, days 0‐20) with no sign of inflammation but detectable T and B cell activation; next, the stage of early arthritis (after the second immunization, days 21‐41), where the first definitive signs of arthritis appear together with autoantibody production; and then the severe late‐stage arthritis (after the third immunization, after day 42), which presents with massive inflammation of the limbs, leading to cartilage and bone destruction and finally ankylosis. The protocols described here provide sufficient information for investigators to use the GIA model to study different aspects of autoimmune arthritis. © 2024 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol: Induction of recombinant human proteoglycan aggrecan‐G1 domain (rhG1)‐induced arthritis (GIA)Support Protocol 1: Production of rhG1‐Xa‐mFc2a fusion protein with CHOK1 mammalian expression systemSupport Protocol 2: Purification of the rhG1‐Xa‐mFc2a fusion protein by affinity chromatographySupport Protocol 3: Preparation of DDA adjuvantSupport Protocol 4: Clinical assessment of arthritisSupport Protocol 5: Measurement of serum antibody levels and cytokinesSupport Protocol 6: Measurement of rhG1‐induced proliferation and cytokine production in spleen cell cultureSupport Protocol 7: Histological assessment of arthritic limbsSupport Protocol 8: Evaluation of arthritis with micro‐computed tomography