Prostate‐derived circulating microRNAs add prognostic value to prostate cancer risk calculators

Author:

Zenner Morgan L.12,Kirkpatrick Brenna12,Leonardo Trevor R.34,Schlicht Michael J.1,Saldana Alejandra Cavazos12,Loitz Candice12,Valyi‐Nagy Klara1,Maienschein‐Cline Mark5,Gann Peter H.12,Abern Michael6,Nonn Larisa12ORCID

Affiliation:

1. Department of Pathology University of Illinois at Chicago Chicago Illinois USA

2. University of Illinois Cancer Center Chicago Illinois USA

3. Department of Microbiology and Immunology University of Illinois Chicago Chicago Illinois USA

4. Department of Periodontics, Center for Wound Healing and Tissue Regeneration University of Illinois Chicago Chicago Illinois USA

5. Research Resource Core Bioinformatics University of Illinois at Chicago Chicago Illinois USA

6. Department of Urology University of Illinois at Chicago Chicago Illinois USA

Abstract

AbstractProstate cancer is the second leading cause of malignancy‐related deaths among American men. Active surveillance is a safe option for many men with less aggressive disease, yet definitively determining low‐risk cancer is challenging with biopsy alone. Herein, we sought to identify prostate‐derived microRNAs in patient sera and serum extracellular vesicles, and determine if those microRNAs improve upon the current clinical risk calculators for prostate cancer prognosis before and after biopsy. Prostate‐derived intracellular and extracellular vesicle‐contained microRNAs were identified by small RNA sequencing of prostate cancer patient explants and primary cells. Abundant microRNAs were included in a custom microRNA PCR panel that was queried in whole serum and serum extracellular vesicles from a diverse cohort of men diagnosed with prostate cancer. The levels of these circulating microRNAs significantly differed between indolent and aggressive disease and improved the area under the curve for pretreatment nomograms of prostate cancer disease risk. The microRNAs within the extracellular vesicles were the most informative and improved the AUC to 0.739 compared to the existing nomogram alone, which has an AUC of 0.561. The microRNAs in the whole serum improved it to AUC 0.675. In summary, quantifying microRNAs circulating in extracellular vesicles is a clinically feasible assay that may provide additional information for assessing prostate cancer risk stratification.

Funder

National Cancer Institute

U.S. Department of Defense

Publisher

Wiley

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3