Affiliation:
1. Institute of Biochemistry Department of Biotechnology and Enzyme Catalysis University of Greifswald Felix-Hausdorff-Straße 4 17487 Greifswald Germany
2. Faculty of Science and Engineering Chemical Technology Engineering and Technology Institute Groningen University of Groningen Nijenborgh 4 9747 AG Groningen Netherlands
Abstract
AbstractCombining solid acid catalysts with enzyme reactions in aqueous environments is challenging because either very acidic conditions inactivate the enzymes, or the solid acid catalyst is neutralized. In this study, Amberlyst‐15 encapsulated in polydimethylsiloxane (Amb‐15@PDMS) is used to deprotect the lignin depolymerization product G−C2 dioxolane phenol in a buffered system at pH 6.0. This reaction is directly coupled with the biocatalytic reduction of the released homovanillin to homovanillyl alcohol by recombinant horse liver alcohol dehydrogenase, which is subsequently acylated by the promiscuous acyltransferase/hydrolase PestE_I208A_L209F_N288A in a one‐pot system. The deprotection catalyzed with Amb‐15@PDMS attains up to 97 % conversion. Overall, this cascade enables conversions of up to 57 %.
Subject
General Energy,General Materials Science,General Chemical Engineering,Environmental Chemistry
Cited by
1 articles.
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