Phospholipase D Immobilization on Lignin Nanoparticles for Enzymatic Transformation of Phospholipids

Author:

Rossato Letizia Anna Maria1ORCID,Morsali Mohammad23ORCID,Ruffini Eleonora1ORCID,Bertuzzi Pietro1,Serra Stefano4ORCID,D'Arrigo Paola14ORCID,Sipponen Mika23ORCID

Affiliation:

1. Department of Chemistry Materials and Chemical Engineering “Giulio Natta” Politecnico di Milano piazza L. da Vinci 32 Milano 20133 Milan Italy

2. Department of Materials and Environmental Chemistry Stockholm University Svante Arrhenius väg 16C SE-10691 Stockholm Sweden

3. Wallenberg Wood Science Center Department of Materials and Environmental Chemistry Stockholm University, SE- 10691 Stockholm Sweden

4. Instituto di Scienze e Tecnologie Chimiche “Giulio Natta” Consiglio Nazionale delle Ricerche (SCITEC-CNR) via Luigi Mancinelli 7 Milano 20131 Italy

Abstract

AbstractLignin nanoparticles (LNPs) are promising components for various materials, given their controllable particle size and spherical shape. However, their origin from supramolecular aggregation has limited the applicability of LNPs as recoverable templates for immobilization of enzymes. In this study, we show that stabilized LNPs are highly promising for the immobilization of phospholipase D (PLD), the enzyme involved in the biocatalytic production of high‐value polar head modified phospholipids of commercial interest, phosphatidylglycerol, phosphatidylserine and phosphatidylethanolamine. Starting from hydroxymethylated lignin, LNPs were prepared and successively hydrothermally treated to obtain c‐HLNPs with high resistance to organic solvents and a wide range of pH values, covering the conditions for enzymatic reactions and enzyme recovery. The immobilization of PLD on c‐HLNPs (PLD‐c‐HLNPs) was achieved through direct adsorption. We then successfully exploited this new enzymatic preparation in the preparation of pure polar head modified phospholipids with high yields (60–90 %). Furthermore, the high stability of PLD‐c‐HLNPs allows recycling for a number of reactions with appreciable maintenance of its catalytic activity. Thus, PLD‐c‐HLNPs can be regarded as a new, chemically stable, recyclable and user‐friendly biocatalyst, based on a biobased inexpensive scaffold, to be employed in sustainable chemical processes for synthesis of value‐added phospholipids.

Funder

VINNOVA

Publisher

Wiley

Subject

General Energy,General Materials Science,General Chemical Engineering,Environmental Chemistry

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