Endoscopy and rectal enema for fecal collection in wild sea turtles (Chelonia mydas, Eretmochelys imbricata) in a field setting

Author:

Kaleel Kristina L.1,Secoura Patricia L.2,Muñoz‐Pérez Juan Pablo3456,Alarcón‐Ruales Daniela345,Vallejo Felipe6,Miranda Cristina6,Lewbart Gregory A.234,Townsend Kathy5,Vaden Shelly L.2

Affiliation:

1. University of Florida College of Veterinary Medicine Gainesville Florida USA

2. The Department of Clinical Sciences North Carolina State College of Veterinary Medicine Raleigh North Carolina USA

3. Colegio de Ciencias Biológicas y Ambientales (COCIBA) Universidad San Francisco de Quito USFQ Quito Ecuador

4. Galápagos Science Center (GSC) Universidad San Francisco de Quito (USFQ) & UNC‐Chapel Hill Galápagos Science Center (GSC) Galápagos Ecuador

5. School of Science, Technology and Engineering University of the Sunshine Coast UniSC Hervey Bay Australia

6. Equilibrio Azul Puerto Lopez Ecuador

Abstract

AbstractForty‐seven free‐ranging sea turtles (46‐ Chelonia mydas, 1‐ Eretmochelys imbricata) were examined via novel use of an endoscopy combined with a rectal enema to obtain large fecal sample volumes. The cloaca was insufflated using an endoscope, after which the bladder and rectum separated, allowing access to the colon. Environmental conditions and location influenced the performance of the procedure initially, but after several attempts the procedure was successfully initiated. In all cases, fecal samples were obtained, and the animals were released to their respective locations. Fecal sample collection using this approach enhances the ability to obtain diagnostic information and perform other scientific analyses of sea turtles.

Publisher

Wiley

Subject

Animal Science and Zoology,General Medicine

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