Affiliation:
1. Institute of Pharmacy Shandong University of Traditional Chinese Medicine Jinan P. R. China
2. National International Joint Research Center for Molecular Chinese Medicine Shanxi University of Chinese Medicine Taiyuan P. R. China
3. College of Pharmacy Shandong University of Traditional Chinese Medicine Jinan China
4. Shandong Cancer Hospital and Institute Shandong First Medical University Jinan P. R. China
5. Shanxi Key Laboratory of Chinese Medicine Encephalopathy Shanxi University of Chinese Medicine Taiyuan P. R. China
Abstract
AbstractIntroductionDajianzhong decoction (DJZD), a classic famous prescription, has a long history of medicinal application. Modern studies have demonstrated its clinical utility in the treatment of postoperative ileus (POI). But none of the current quality evaluation methods for this compound is associated with efficacy.ObjectivesThis study aimed to identify the quality markers (Q‐Markers) connected to the treatment of POI in DJZD.MethodologyUltra‐performance liquid chromatography quadrupole Exactive Orbitrap mass spectrometry (UPLC‐Q‐Exactive Orbitrap‐MS) was used to identify the main constituents in DJZD. Based on the qualitative results obtained by fingerprinting, chemical pattern recognition (CPR) was used to analyse the key components affecting the quality and finally to establish the network of the active ingredients in DJZD with POI.ResultsA total of 64 chemical components were detected. After fingerprint analysis, 13 common peaks were identified. The fingerprint similarity of 15 batches of samples ranged from 0.860 to 1.000. CPR analysis was able to categorically classify 15 batches of DJZD into two groups. And gingerenone A, methyl‐6‐gingerdiol, 6‐gingerol, and hydroxy‐β‐sanshool contributed to their grouping. Twelve common components interact with the therapeutic targets for treating POI. In addition, the mechanism of this prescription for treating POI may be related to the jurisdiction of the neurological system, the immunological system, and the inflammatory response.ConclusionsThis integrated approach can accurately assess and forecast the quality of DJZD, presume the Q‐Markers of DJZD for POI, and lay the foundation for studying the theoretical underpinnings and exploring the mechanism of DJZD in the treatment of POI.
Funder
National Major Science and Technology Projects of China
Subject
Complementary and alternative medicine,Drug Discovery,Plant Science,Molecular Medicine,General Medicine,Biochemistry,Food Science,Analytical Chemistry
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