N‐Terminal Protein Labeling with N ‐Hydroxysuccinimide Esters and Microscale Thermophoresis Measurements of Protein‐Protein Interactions Using Labeled Protein-Reference-Cited by-同舟云学术

N‐Terminal Protein Labeling with N ‐Hydroxysuccinimide Esters and Microscale Thermophoresis Measurements of Protein‐Protein Interactions Using Labeled Protein

Published:2021-01 Issue:1 Volume:1 Page:
ISSN:2691-1299
Container-title:Current Protocols
language:en
Short-container-title:Current Protocols

Author:

Jiang Hanjie¹²,Cole Philip A.¹

Affiliation:

1. Division of Genetics, Brigham and Women's Hospital, Department of Medicine and Biological Chemistry and Molecular Pharmacology Harvard Medical School Boston Massachusetts

2. Department of Pharmacology and Molecular Sciences Johns Hopkins School of Medicine Baltimore Maryland

Funder

National Science Foundation

Publisher

Wiley

Subject

Medical Laboratory Technology,Health Informatics,General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/cpz1.14

Reference67 articles.

1. The use of activated esters in peptide synthesis

2. Site-Specific N- and C-Terminal Labeling of a Single Polypeptide Using Sortases of Different Specificity

3. Thermophoresis for characterizing biomolecular interaction

4. Label-free quantification of membrane-ligand interactions using backscattering interferometry

5. New insights into ubiquitin E3 ligase mechanism

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