Affiliation:
1. Surgery Department Faculty of Medical Sciences and Health Pontifical Catholic University of São Paulo Postgraduate Program in Biomaterials and Regenerative Medicine (PPBMR) Laboratory of Biomaterials Sorocaba 18030–070 Brazil
2. Center of Sciences and Technology for Sustainability Federal University of São Carlos Postgraduate Program in Biotechnology and Environmental Monitoring Sorocaba 18052–780 Brazil
3. Biotechnology Center Butantan Institute Laboratory of Vaccine Development Sorocaba 05508‐040 Brazil
4. Department of Functional and Structural Biology University of Campinas Institute of Biology Laboratory of Bone Regeneration and Plasticity São Paulo 13083–970 Brazil
Abstract
AbstractThe skin, the human body's largest organ, possesses a protective barrier that renders it susceptible to various injuries, including burns. Following burn trauma, the inflammatory process triggers both innate and adaptive immune responses, leading to the polarization of macrophages into two distinct phenotypes: the pro‐inflammatory M1 and the anti‐inflammatory M2. This dual response sets the stage for wound healing and subsequent tissue regeneration. Contributing to this transition from M1 to M2 polarization are human adipose‐derived stem cells (ASCs), which employ paracrine signaling and inflammation suppression to enhance the remodeling phase. ASCs, when combined with biocompatible polymers, can be integrated into functional scaffolds. This study introduces an 1‐ethyl‐3‐(3‐dimethylaminopropyl)carbodiimide‐crosslinked (EDC‐crosslinked) collagen‐hyaluronic acid (Col‐HA) scaffold assembled with ASCs, designed as a natural biomaterial device to modulate macrophage behavior in vitro under co‐culture conditions. This innovation aims to improve wound healing processes. The EDC‐crosslinked Col‐HA scaffold favored the release of anti‐inflammatory cytokines by ASCs, which indicated the M2 prevalence. In tissue engineering, a critical objective lies in the development of functional biomaterials capable of guiding specific tissue responses, notably the control of inflammatory processes. Thus, this research not only presents original findings but also points toward a promising avenue within regenerative medicine.
Funder
Fundação de Amparo à Pesquisa do Estado de São Paulo
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Financiadora de Estudos e Projetos
Subject
Materials Chemistry,Polymers and Plastics,Biomaterials,Bioengineering,Biotechnology