Affiliation:
1. Department of Biomedical Engineering The Chinese University of Hong Kong Hong Kong SAR China
2. Hong Kong Branch of CAS Center for Excellence in Animal Evolution and Genetics The Chinese University of Hong Kong Hong Kong SAR China
3. Ministry of Education Key Laboratory of Regeneration Medicine Hong Kong SAR China
4. Centre for Novel Biomaterials The Chinese University of Hong Kong Hong Kong SAR China
Abstract
AbstractCurrently available deformability cytometry, combining microfluidics with the bright‐field high‐speed imaging system, has allowed label‐free mechanical characterizations of cells at a rate of hundreds of cells per second. However, the poor contrast between the signal and background under bright‐field imaging, especially for abnormal cells or cells treated with drugs, may produce artifacts during the recognition of cellular contours and subsequently bias the analysis of cellular deformability. In this study, a quantitative phase deformability cytometry (QPDC), constructed from a quantitative phase imaging system, is demonstrated with an improved signal‐to‐noise ratio of the acquired images and mitigated image artifacts, allowing a truthful illustration of cellular contours, especially for cells subjected to actin depolymerization. We have also demonstrated that the phase value extracted from captured images may provide an additional biophysical characteristic of cells. The presented QPDC platform is therefore expected to complement the existing deformability cytometry and expand the capacity of label‐free characterization of cells for biological and clinical applications.
Subject
Biomedical Engineering,Biomaterials
Cited by
2 articles.
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