Selectable high-yield recombinant protein production in human cells using a GFP/YFP nanobody affinity support

Author:

Schellenberg Matthew J.1,Petrovich Robert M.1,Malone Christine C.1,Williams R. Scott1

Affiliation:

1. Structural Cell Biology Group, Genome Integrity and Structural Biology Laboratory; National Institute of Environmental Health Sciences, US National Institutes of Health, Department of Health and Human Services; Research Triangle Park North Carolina 27709

Funder

National Institutes of Health

National Institute of Environmental Health Sciences

Publisher

Wiley

Subject

Molecular Biology,Biochemistry

Reference27 articles.

1. Recombinant protein expression in Escherichia coli: advances and challenges;Rosano;Front Microbiol,2014

2. Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production;Ahmad;Appl Microbiol Biotechnol,2014

3. Different expression systems for production of recombinant proteins in Saccharomyces cerevisiae;Liu;Biotechnol Bioeng,2012

4. Protein production using the baculovirus-insect cell expression system;Contreras-Gómez;Biotechnol Prog,2014

5. Recombinant protein expression for structural biology in HEK 293F suspension cells: a novel and accessible approach;Portolano;J Vis Exp,2014

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