The Mythimna separata general odorant binding protein 2 (MsepGOBP2) is involved in the larval detection of the sex pheromone (Z)‐11‐hexadecenal

Author:

Tian Zhen1ORCID,Li Ruichi1,Cheng Shichang2,Zhou Tong2,Liu Jiyuan1ORCID

Affiliation:

1. Key Laboratory of Plant Protection Resources and Pest Management of the Ministry of Education, College of Plant Protection Northwest A&F University Yangling China

2. College of Horticulture and Plant Protection Yangzhou University Yangzhou China

Abstract

AbstractBACKGROUNDMythimna separata is a notorious pest causing crop damages at the larval stage. Gaining insight into larval olfaction mechanisms would provide knowledge for olfaction‐based management of M. separata larvae.RESULTSIn the present research, (Z)‐11‐hexadecenal (Z11‐16: Ald), a major component of M. separata sex pheromone, was found to attract early‐instar larvae of M. separata in a food context. Using a fluorescent binding assay, we found that M. separata general odorant binding protein 2 (MsepGOBP2) exhibited high binding affinity to Z11‐16: Ald. Further, silencing of MsepGOBP2 resulted in a sharp reduction of the response to Z11‐16: Ald, which could not be mitigated by increasing the concentration of Z11‐16: Ald. Additionally, we employed molecular dynamics‐based approaches to unravel the interaction details between MsepGOBP2 and Z11‐16: Ald, specifically the binding of Z11‐16: Ald to MsepGOBP2.CONCLUSIONZ11‐16: Ald is attractive to early‐instar larvae of M. separata, and MsepGOBP2 is identified to be indispensable in the larval detection of Z11‐16: Ald. These results could aid in the development of olfaction‐based methods for controlling M. separata in the larval stage. © 2023 Society of Chemical Industry.

Funder

Chinese Universities Scientific Fund

National Natural Science Foundation of China

Natural Science Foundation of Jiangsu Province

Publisher

Wiley

Subject

Insect Science,Agronomy and Crop Science,General Medicine

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