The gene AccCyclin H mitigates oxidative stress by influencing trehalose metabolism in Apis cerana cerana

Author:

Peng Hongyan1,Guo Dezheng1,Peng Hongmei1,Guo Hengjun1,Wang Hongfang2,Wang Ying2,Xu Baohua2,Gao Aiying3,Liu Zhenguo2,Guo Xingqi1ORCID

Affiliation:

1. State Key Laboratory of Crop Biology, College of Life Sciences Shandong Agricultural University Taian PR China

2. Key Laboratory of Efficient Utilization of Non‐grain Feed Resources (Co‐construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology Shandong Agricultural University Taian PR China

3. Taian Institute for Food and Drug Control (Taian Fiber Inspection Institute) Taian PR China

Abstract

AbstractBACKGROUNDEnvironmental stress can induce oxidative stress in Apis cerana cerana, leading to cellular oxidative damage, reduced vitality, and even death. Currently, owing to an incomplete understanding of the molecular mechanisms by which A. cerana cerana resists oxidative damage, there is no available method to mitigate the risk of this type of damage. Cyclin plays an important role in cell stress resistance. The aim of this study was to explore the in vivo protection of cyclin H against oxidative damage induced by abiotic stress in A. cerana cerana and clarify the mechanism of action. We isolated and identified the AccCyclin H gene in A. cerana cerana and analysed its responses to different exogenous stresses.RESULTSThe results showed that different oxidative stressors can induce or inhibit the expression of AccCyclin H. After RNA‐interference‐mediated AccCyclin H silencing, the activity of antioxidant‐related genes and related enzymes was inhibited, and trehalose metabolism was reduced. AccCyclin H gene silencing reduced A. cerana cerana high‐temperature tolerance. Exogenous trehalose supplementation enhanced the total antioxidant capacity of A. cerana cerana, reduced the accumulation of oxidants, and improved the viability of A. cerana cerana under high‐temperature stress.CONCLUSIONOur findings suggest that trehalose can alleviate adverse stress and that AccCyclin H may participate in oxidative stress reactions by regulating trehalose metabolism. © 2023 Society of Chemical Industry.

Publisher

Wiley

Subject

Nutrition and Dietetics,Agronomy and Crop Science,Food Science,Biotechnology

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3