Affiliation:
1. Florida Museum of Natural History University of Florida Gainesville 32611 Florida USA
2. Department of Biology University of Florida Gainesville 32611 Florida USA
3. School of Integrative Plant Science Cornell University Ithaca 14850 New York USA
4. Department of Ecology and Evolution University of Chicago Chicago 60637 Illinois USA
5. Department of Biology The College of Idaho Caldwell 83605 Idaho USA
Abstract
AbstractPremiseTraditional methods of ploidal‐level estimation are tedious; using DNA sequence data for cytotype estimation is an ideal alternative. Multiple statistical approaches to leverage sequence data for ploidy inference based on site‐based heterozygosity have been developed. However, these approaches may require high‐coverage sequence data, use inappropriate probability distributions, or have additional statistical shortcomings that limit inference abilities. We introduce nQuack, an open‐source R package that addresses the main shortcomings of current methods.Methods and ResultsnQuack performs model selection for improved ploidy predictions. Here, we implement expectation maximization algorithms with normal, beta, and beta‐binomial distributions. Using extensive computer simulations that account for variability in sequencing depth, as well as real data sets, we demonstrate the utility and limitations of nQuack.ConclusionsInferring ploidy based on site‐based heterozygosity alone is difficult. Even though nQuack is more accurate than similar methods, we suggest caution when relying on any site‐based heterozygosity method to infer ploidy.
Cited by
2 articles.
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