Affiliation:
1. Department of Medical BioSciences Radboud University Medical Center Nijmegen the Netherlands
2. Department of Pulmonary Diseases Radboud University Medical Center Nijmegen the Netherlands
3. Data Science Institute for Computing and Information Sciences Radboud University Nijmegen the Netherlands
4. Division of Immunotherapy Oncode Institute Radboud University Medical Center Nijmegen the Netherlands
5. Department of Pathology Radboud University Medical Center Nijmegen the Netherlands
Abstract
AbstractDendritic cells (DCs) are essential in antitumor immunity. In humans, three main DC subsets are defined: two types of conventional DCs (cDC1s and cDC2s) and plasmacytoid DCs (pDCs). To study DC subsets in the tumor microenvironment (TME), it is important to correctly identify them in tumor tissues. Tumor‐derived DCs are often analyzed in cell suspensions in which spatial information about DCs which can be important to determine their function within the TME is lost. Therefore, we developed the first standardized and optimized multiplex immunohistochemistry panel, simultaneously detecting cDC1s, cDC2s, and pDCs within their tissue context. We report on this panel's development, validation, and quantitative analysis. A multiplex immunohistochemistry panel consisting of CD1c, CD303, X‐C motif chemokine receptor 1, CD14, CD19, a tumor marker, and DAPI was established. The ImmuNet machine learning pipeline was trained for the detection of DC subsets. The performance of ImmuNet was compared with conventional cell phenotyping software. Ultimately, frequencies of DC subsets within several tumors were defined. In conclusion, this panel provides a method to study cDC1s, cDC2s, and pDCs in the spatial context of the TME, which supports unraveling their specific roles in antitumor immunity.
Funder
Hanarth Fonds
Nederlandse Organisatie voor Wetenschappelijk Onderzoek
Health~Holland
Subject
Immunology,Immunology and Allergy
Cited by
1 articles.
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