Single‐cell RNA sequencing of cells from fresh or frozen tissue reveals a signature of freezing marked by heightened stress and activation-Reference-Cited by-同舟云学术

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Single‐cell RNA sequencing of cells from fresh or frozen tissue reveals a signature of freezing marked by heightened stress and activation

Published:2024-02-02 Issue:4 Volume:54 Page:
ISSN:0014-2980
Container-title:European Journal of Immunology
language:en
Short-container-title:Eur J Immunol

Author:

Stamper Christopher T.¹^ORCID,Marchalot Anne¹,Tibbitt Christopher A.¹²,Weigel Whitney¹,Jangard Mattias³,Theorell Jakob¹⁴,Mjösberg Jenny¹²^ORCID

Affiliation:

1. Department of Medicine Huddinge Center for Infectious Medicine Karolinska Institutet Karolinska University Hospital Huddinge Stockholm Sweden

2. Clinical Lung and Allergy Research Unit Medical Unit for Lung and Allergy Diseases Karolinska University Hospital Huddinge Stockholm Sweden

3. ENT Unit Sophiahemmet University Research Laboratory and Sophiahemmet Hospital Stockholm Sweden

4. Department of Neurology Karolinska University Hospital Stockholm Sweden

Funder

Familjen Erling-Perssons Stiftelse

Knut och Alice Wallenbergs Stiftelse

Göran Gustafssons Stiftelse för Naturvetenskaplig och Medicinsk Forskning

Vetenskapsrådet

H2020 European Research Council

Karolinska Institutet

Cancerfonden

Hjärt-Lungfonden

Publisher

Wiley

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/eji.202350660

Reference14 articles.

1. Cryopreservation Preserves Cell-Type Composition and Gene Expression Profiles in Bone Marrow Aspirates From Multiple Myeloma Patients

2. RNA-seq: impact of RNA degradation on transcript quantification

3. Multiple freeze-thaw cycles lead to a loss of consistency in poly(A)-enriched RNA sequencing

4. Effects of Cryopreservation and Thawing on Single-Cell Transcriptomes of Human T Cells

5. Long Term Storage of Dry versus Frozen RNA for Next Generation Molecular Studies

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