Preparative separation of the constituents from Ginkgo biloba L. leaves by a combination of multi‐stage solvent extraction and countercurrent chromatography

Author:

Liu Zhuo1,Tian Peng1,Li Luqi2,Guo Jinxing1,Zhou Yi1,Liu Shuhui1ORCID,Yang Zhi1ORCID

Affiliation:

1. College of Chemistry & Pharmacy Northwest A&F University Yangling China

2. Life Science Research Core Services Northwest A&F University Yangling China

Abstract

In this study, we employed a combination approach for the preparative separation of constituents from Ginkgo biloba L. leaves. It involved multi‐stage solvent extractions utilizing two‐phase multi‐solvent systems and countercurrent chromatography (CCC) separations using three different solvent systems. The n‐heptane/ethyl acetate/water (1:1:2, v/v) and n‐heptane/ethyl acetate/methanol/water (HepEMWat, 7:3:7:3, v/v) solvent systems were screened out as extraction systems. The polarities of the upper and lower phases in the multi‐solvent systems were adjustable, enabling the effectively segmented separation of complex constituents in G. biloba L. The segmented products were subsequently directly utilized as samples and separated using CCC with the solvent systems acetate/n‐butanol/water (4:1:5, v/v), HepEMWat (5:5:5:5, v/v), and HepEMWat (9:1:9:1, v/v), respectively. As a result, a total of 11 compounds were successfully isolated and identified from a 2 g methanol extract of G. biloba L through two‐stage extraction and three CCC separation processes; among them, nine compounds exhibited high‐performance liquid chromatography purity exceeding 85%.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Shaanxi Province

Publisher

Wiley

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