Ultra‐performance liquid chromatography‐quadrupole time‐of‐flight mass combined with UNIFI to study the mechanism of Tao Hong Si Wu Decoction in the treatment of postpartum blood stasis

Author:

Zhao Mengdie1ORCID,Xia Wenwen1,Zhang Peiliang1,Xie Qingqing1,Mu Wenyu1,Tang Linfeng1,Liu Zhuqing123,Han Lan123ORCID,Peng Daiyin123

Affiliation:

1. School of Pharmacy Anhui University of Chinese Medicine Hefei China

2. Anhui Province Key Laboratory of Chinese Medicinal Formula Hefei China

3. Xin'an Medicine Key Laboratory of Chinese Ministry of Education, Anhui University of Chinese Medicine Hefei China

Abstract

Postpartum hemorrhage can lead to a variety of maternal complications. Tao Hong Si Wu Decoction (THSWD) is a traditional Chinese medicine used for treating gynecological diseases. However, the active ingredients of THSWD and its pharmacological mechanism of treatment for postpartum blood stasis still remained unclear. In this study, 201 components were identified in THSWD ethanol extract using ultra‐performance liquid chromatography coupled with quadrupole‐time‐of‐flight mass spectrometry, including 59 terpenoids and volatile oil, 61 Phenylpropanoids, 41 flavonoids, 22 alkaloids, and other 18 components. A total of 45 active compounds were identified in the blood and 33 active compounds were identified in the uterine. Taking the common components into the blood and into the uterus combined with network pharmacology. It was demonstrated that the active compounds can bind to the core target with good affinity through molecular docking. The results of this study will provide a reference for the quality control and pharmacodynamic material base research of THSWD.

Publisher

Wiley

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