Identification of bibenzyls and evaluation of imitative wild planting techniques in Dendrobium officinale by HPLC‐ESI‐MSn

Author:

Meng Yuanjun1,Wang Yawen1,Zhang Lin1,Li Jinyan2,Hu Li23,Wu Zhanghua23,Yang Li'e4,Wei Gang2,Huang Yuechun14ORCID

Affiliation:

1. The First College of Clinical Medicine Guangzhou University of Chinese Medicine Guangzhou 510405 China

2. School of Pharmaceutical Science Guangzhou University of Chinese Medicine Guangzhou 510006 China

3. Shaoguan Institute of Danxia Dendrobium Officinale (SIDDO) Shaoguan 512005 China

4. The First Affiliated Hospital Guangzhou University of Chinese Medicine Guangzhou 510405 China

Abstract

AbstractDendrobium officinale is a traditional Chinese herb with beneficial properties. Modern pharmacological studies show that bibenzyl is one of the antitumor active ingredients, but there is no effective quality control method for identifying ingredients. In this study, the composition of bibenzyls in Dendrobium officinale was studied by high‐performance liquid chromatography coupled with electrospray ionization multistage mass spectrometry (HPLC‐ESI‐MSn). A total of nine isolated bibenzyls and their glycosides, 22 bis (bibenzyls), and two phenylpropanol bibenzyl derivatives were identified. The results of HPLC characteristic chromatogram analysis and statistical analysis showed that the relative content of bibenzyls in wild imitation cultivation of samples had been significantly higher than that in greenhouse cultivation. In addition, the relative content of bibenzyls increased with the growth of the original plant. This study provided a scientific reference for controlling the quality of bibenzyls in Dendrobium officinale, developing the cultivation technology and improving the quality of Dendrobium officinale.Highlights HPLC‐ESI‐MS/MS method for the analysis of bibenzyls and bis (bibenzyls) in Dendrobium officinale. Easy‐to‐use method facilitating rapid measurement of large sample quantities. The method requires only small volumes of samples for the analysis. Applicable for the establishment of Chinese medicine studies and the quality control standard of Chinese herbs.

Publisher

Wiley

Subject

Spectroscopy

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