Affiliation:
1. Department of Research and Development Qingdao Bioman Biomedical Technology Co., LTD Qingdao China
2. Department of Research and Development Shanghai life Biomedical Technology Co., LTD Shanghai China
3. Department of Student Affairs The 2nd Affiliated Hospital of Harbin Medical University Harbin China
4. Precision Medicine Center of Oncology The Affiliated Hospital of Qingdao University Qingdao China
5. Department of Emergency Medicine and Intensive Care Shanghai Songjiang District Central Hospital Shanghai China
Abstract
AbstractDespite substantial advances in cancer biology and treatment, the clinical outcomes of patients with lung cancer remain unsatisfactory. The tumor microenvironment (TME) is a potential target. Using single‐cell RNA sequencing, we could distinguish eight distinct cell types in the lung cancer microenvironment, demonstrating substantial intratumoral heterogeneity in 19 different lung cancer tumor samples. Through the re‐dimensional grouping of cancer‐associated fibroblasts (CAFs), myeloid cells, epithelial cells, natural killer (NK) cells, and T cells, the difference in the TME of lung cancer was revealed. We discovered SFTPB, SFN, and KRT8 as possible predictive biomarkers for lung cancer by assessing the gene expression patterns in epithelial cells. Examining cell‐to‐cell communications showed a robust association between the quantity of matrix CAFs, epithelial cells, and macrophages in the thrombospondin signaling pathway. Additionally, we found that the amyloid precursor protein signaling pathway primarily originated from the matrix, and inflammatory cancer‐associated endothelial and fibroblast cells showed a co‐expression relationship with myeloid cells and B cells. Through cell‐to‐cell correlation analysis, we found positive regulation between NK cells, regulatory T cells, GZMB‐CD8 T cells, and GZMK‐CD8 T cells, which could play a role in developing immune TMEs. These findings support studies on cancer heterogeneity and add to our understanding of lung cancer's cellular microenvironment.
Subject
Health, Toxicology and Mutagenesis,Management, Monitoring, Policy and Law,Toxicology,General Medicine
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献