Genotoxicity of bisphenol AF in rats: Detrimental to male reproductive system and probable stronger micronucleus induction potency than BPA

Author:

Zhu Xia12ORCID,Cao Li13,Liu Yufei13,Tang Xinyao13,Miao Yeqiu13,Zhang Jing13,Zhang Lishi13,Jia Zhenchao4,Chen Jinyao13ORCID

Affiliation:

1. West China School of Public Health and West China Fourth Hospital Sichuan University Chengdu China

2. Second People's Hospital of Yibin City Yibin China

3. Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province Chengdu China

4. Department of Prevention and Health Care Sichuan University Hospital of Sichuan University Chengdu China

Abstract

AbstractBisphenol AF (BPAF), as one of structural analogs of BPA, has been increasingly used in recent years. However, limited studies have suggested its adverse effects similar to or higher than BPA. In order to explore the general toxicity and genotoxicity of subacute exposure to BPAF, the novel 28‐day multi‐endpoint (Pig‐a assay + micronucleus [MN] test + comet assay) genotoxicity evaluation platform was applied. Male rats were randomly distributed into seven main experimental groups and four satellite groups. The main experimental groups included BPAF‐treated groups (0.5, 5, and 50 μg/kg·bw/d), BPA group (10 μg/kg·bw/d), two solvent control groups (PBS and 0.1% ethanol/99.9% oil), and one positive control group (N‐ethyl‐N‐nitrosourea, 40 mg/kg bw). The satellite groups included BPAF high‐dose recovery group (BPAF‐HR), oil recovery group (oil‐R), ENU recovery group (ENU‐R), and PBS recovery group (PBS‐R). All groups received the agents orally via gavage for 28 consecutive days, and satellite groups were given a recovery period of 35 days. Among all histopathologically examined organs, testis and epididymis damage was noticed, which was further manifested as blood‐testis barrier (BTB) junction protein (Connexin 43 and Occludin) destruction. BPAF can induce micronucleus production and DNA damage, but the genotoxic injury can be repaired after the recovery period. The expression of DNA repair gene OGG1 was downregulated by BPAF. To summarize, under the design of this experiment, male reproductive toxicity of BPAF was noticed, which is similar to that of BPA, but its ability to induce micronucleus production may be stronger than that of BPA.

Funder

National Natural Science Foundation of China

Sichuan University

Department of Science and Technology of Sichuan Province

Publisher

Wiley

Subject

Toxicology

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