Affiliation:
1. BIOFABICS Rua Alfredo Allen 455 4200-135 Porto Portugal
2. Institute of Chemical Technologies and Analytics Vienna University of Technology Getreidemarkt 9/164 1060 Vienna Austria
3. Institute of Experimental Medicine of the Czech Academy of Sciences Vídeňská 1083 14220 Prague Czechia
4. Institute of Pharmaceutical Sciences University of Graz Universitaetsplatz 1 8010 Graz Austria
5. InoCure s.r.o. Politických vězňů 935/13 11000 Praha 1 Prague Czech Republic
6. Fraunhofer Institute for Biomedical Engineering IBMT Joseph-von-Fraunhofer-Weg 1 66280 Sulzbach/Saar Germany
Abstract
Herein, intestinal, skin, and pulmonary in vitro tissue models based on electrospun membranes of poly(ε‐caprolactone) (PCL) and cellulose acetate (CA), cellulose acetate phthalate (CAP), ethylcellulose (EC), or methylcellulose (MC) are presented. Physicochemical characterization and biocompatibility analyses of the scaffolds are carried out using colorectal adenocarcinoma cells (intestine), keratinocytes and fibroblasts (skin), and bronchial and alveolar epithelial cells (lung). PCL, PCL:CA, and PCL:EC are composed of nanofibers, whereas PCL:CAP and PCL:MC scaffolds comprise a combination of micro‐ and nanofibers. PCL, PCL:CA, PCL:CAP, and PCL:EC samples demonstrate water contact angles greater than 90° and are, therefore, hydrophobic, while PCL:MC mats display a hydrophilic behavior. In intestinal models, cells adhere and proliferate on all scaffolds; in turn, studies with skin cell models reveal that PCL:CA and PCL:CAP blends outperform all other substrates. Lung cell models show that, while 16HBE cells adhere to and proliferate in PCL, PCL:CA, PCL:EC, and PCL:MC scaffolds, A549 cells only have the same biological response on PCL, PCL:CA, and PCL:MC. In summary, all fibrous meshes prepared are biocompatible toward most cell types tested, thus suggesting the potential of PCL‐cellulose derivative blends as substrates suitable for in vitro epithelial tissue modeling and toxicity screening.
Funder
H2020 Marie Skłodowska-Curie Actions