Unraveling the Relevance of Tissue‐Specific Decellularized Extracellular Matrix Hydrogels for Vocal Fold Regenerative Biomaterials: A Comprehensive Proteomic and In Vitro Study

Author:

Brown Mika1ORCID,Zhu Shirley2,Taylor Lorne3,Tabrizian Maryam145ORCID,Li-Jessen Nicole Y. K.1678ORCID

Affiliation:

1. Department of Biomedical Engineering McGill University 3655 Promenade Sir-William-Osler, Room 1003 Montreal QC H3A 1A3 Canada

2. Department of Microbiology and Immunology McGill University 2001 McGill College Ave, 8th Floor Montreal QC H3A 1G1 Canada

3. The Proteomics Platform McGill University Health Center 1001 Decarie Boulevard Montreal Suite E01.5056 Montreal QC H4A 3J1 Canada

4. Department of Bioengineering McGill University 740 Avenue Dr. Penfield, Room 4300 Montreal QC H3A 0G1 Canada

5. Faculty of Dentistry McGill University 740 Avenue Dr. Penfield, Room 4300 Montreal QC H3A 0G1 Canada

6. School of Communication Sciences and Disorders McGill University 2001 McGill College Ave, 8th Floor Montreal QC H3A 1G1 Canada

7. Department of Otolaryngology - Head and Neck Surgery McGill University 2001 McGill College Ave, 8th Floor Montreal QC H3A 1G1 Canada

8. Research Institute of McGill University Health Center McGill University 2001 McGill College Ave, 8th Floor Montreal QC H3A 1G1 Canada

Abstract

Decellularized extracellular matrix (dECM) is a promising material for tissue engineering applications. Tissue‐specific dECM is seen as a favorable material that recapitulates a native‐like microenvironment for cellular remodeling. However, the minute quantity of dECM derivable from small organs like the vocal fold (VF) hampers manufacturing scalability. Small intestinal submucosa (SIS), a commercial product with proven regenerative capacity, may be a viable option for VF applications. This study aims to compare SIS and VF dECM hydrogels with respect to protein content and functionality using mass spectrometry‐based proteomics and in vitro studies. Proteomic analysis reveals that VF and SIS dECM share 75% of core matrisome proteins. Although VF dECM proteins have greater overlap with native VF, SIS dECM shows less cross‐sample variability. Following decellularization, significant reductions of soluble collagen (61%), elastin (81%), and hyaluronan (44%) are noted in VF dECM. SIS dECM contains comparable elastin and hyaluronan but 67% greater soluble collagen than VF dECM. Cells deposit more neo‐collagen on SIS than VF‐dECM hydrogels, but comparable neo‐elastin (≈50 μg scaffold−1) and neo‐hyaluronan (≈6 μg scaffold−1). Overall, SIS dECM possesses a reasonably similar proteomic profile and regenerative capacity to VF dECM. SIS dECM is a promising alternative for dECM‐derived biomaterials for VF regeneration.

Funder

National Institute on Deafness and Other Communication Disorders

Natural Sciences and Engineering Research Council of Canada

Canada Research Chairs

Publisher

Wiley

Subject

General Medicine

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