Affiliation:
1. Integrated Hospital of Traditional Chinese Medicine Southern Medical University Guangzhou China
2. Cancer Center Southern Medical University Guangzhou China
3. Department of Head and Neck Surgery Affiliated Cancer Hospital and Institute of Guangzhou Medical University Guangzhou China
4. Department of Hepatobiliary Surgery, The Third Affiliated Hospital Sun Yat‐sen University Guangzhou China
5. Department of Pathology Affiliated Cancer Hospital and Institute of Guangzhou Medical University Guangzhou China
Abstract
AbstractPapillary thyroid cancer (PTC) is a prevalent malignancy worldwide. Spleen tyrosine kinase (SYK) is a crucial enzyme that participates in various biological processes, including cancer progression. This study aims to uncover the biological function of SYK in PTC. SYK expression patterns in PTC were evaluated using quantitative real time polymerase chain reaction (qRT‐PCR), immunohistochemistry (IHC), and western blot. Cell function assays were performed to assess the effects of SYK on PTC. Bioinformatics analysis was conducted to identify intriguing microRNA (miRNA) and circular RNA (circRNA). Dual‐Luciferase Reporter or RNA immunoprecipitation assays were used to investigate the correlation among SYK, miR‐377‐3p, and hsa_circ_0006417. SYK was upregulated in PTC. Overexpression of SYK exhibited a positive correlation with tumor size, lymph node metastasis, and unfavorable disease‐free survival. Functional assays revealed that SYK exerted tumorigenic effect on PTC cells through mTOR/4E‐BP1 pathway. Mechanistically, hsa_circ_0006417 and miR‐377‐3p regulated SYK expression, offering modulating its tumor‐promoting effects. Collectively, SYK acts as an oncogene in PTC through mTOR/4E‐BP1 pathway, which is regulated by the hsa_circ_0006417/miR‐377‐3p axis, thereby providing a potential alternative for PTC treatment.
Funder
Guangzhou Municipal Science and Technology Project
National Natural Science Foundation of China
Natural Science Foundation of Guangdong Province
Subject
Health, Toxicology and Mutagenesis,Management, Monitoring, Policy and Law,Toxicology,General Medicine