Affiliation:
1. Department of Radiology, The Sixth Hospital of Wuhan Affiliated Hospital of Jianghan University Wuhan 430000 China
2. Department of Radiotherapy People’s Hospital of Ningxia Hui Autonomous Region Yinchuan 750002 China
3. Department of Mechanical and Bio Mechanical Engineerig, Urmia Branch Islamic Azad University Urmia Iran
Abstract
The report focuses on the preparation of chitosan mediated gold nanoparticles (Au@CS NPs) and theirapplication in radiotherapy. It highlights the use of gold nanoparticles as a way to enhance the efficacy of radiation therapy in cancer treatment. The process of fabrication of these gold nanoparticles using CS as both a stabilizing and reducing agent is detailed. CS, a biopolymer derived from chitin, is considered biocompatible and biodegradable and has been used largely in biomedicine. The properties of gold nanoparticles stabilized by CS were analyzed using various techniques such as FESEM, STEM, DLS, zeta potential, and UV–Vis. Finally, the in vitro assays were conducted to study the radioenhancement effect of Au@CS NPs on breast cancer cell line (4T1) as a radiosensitizer agent through the cell viability and reactive oxygen species (ROS) generation assays. The results show that Au@CS NPs could produce ROS within cancer cells and enhance sensitization of cancer cells against X‐ray.